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merge IgH productive/sterile transcript count into Seurat Object

mergeIgHCountsToSeurat.Rd

mergeIgHCountsToSeurat will generate a new Assay in the given Seurat Object which contains the count matrix for productive/sterile transcripts

Usage

mergeIgHCountsToSeurat(igh_counts, SeuratObj, assay_name = "IGHC")

Arguments

igh_counts

count matrix of productive/sterile transcripts after ensuring cell names/barcodes match with the supplied Seurat Object (i.e. after running the repairBarcode function)

SeuratObj

Seurat object on to the productive/sterile count matrix will be attached

assay_name

name of the Seurat Assay holding the productive/sterile transcript count matrix. (Default: "IGHC")

Value

A Seurat object identical to SeuratObj with a new Assay with name given by assay_name, containing the productive/sterile transcript count matrix igh_counts.

Details

mergeIgHCountsToSeurat expects a count matrix with matching cell names/barcodes with the Seurat Object (i.e. output from the repairBarcode of sciCSR). It will create a new Assay that stores this count matrix.